DESCRIPTION:
Protein Purification
Cost-effective and innovative chromatographic purification platforms
Many tools and techniques are available today for protein purification. Development of a purification process requires an analysis of various strategies to isolate a protein of interest. Protein purification from a complex sample, such as cell culture or serum, requires more than one chromatographic step. Typically the first step is ion exchange (IEX) or mixed-mode chromatography to fractionate the sample and yield a subpopulation of proteins containing the target molecule. This is followed by subsequent chromatographic steps to further fractionate the sub-population until a desired purity level is reached. These steps can include affinity purification and size exclusion chromatography, as well as IEX or mixed-mode chromatography to isolate the target protein.
Each step in the purification process requires optimization in order to maximize yield and purity of the target protein. Thus, small-scale purification experiments are used during the development and optimization phase to preserve precious sample while providing crucial information. The methods demonstrate the use of AcroPrep™ Advance 96-well filter plates combined with Pall chromatography media as an efficient tool for fractionation of small-volume protein samples. The format can be used to develop protein purification strategies and/or as a platform for moderate to high throughput needs.
AcroPrep Advance 96-well filter plates offer improved consistency and reproducibility;
Well design increases well-to-well consistency for sample processing and recovery.
Outlet tips minimize the formation of hanging drops, reducing the chance of cross contamination following filtration.
Optimized membrane configurations for assays, such as multiplexing.
Individually sealed membranes and biologically inert housing minimizes the possibility of sample cross-contamination or loss.
Designed to meet SBS/ANSI guidelines, ensuring compatibility with standard processing equipment.
Smooth top surface, textured window for labelling, and A1 corner notch allow for easy identification.