Formulation: Liquid
Activity: >2500 IU beta I/vial >1000 IU beta II/vial
Sales Unit: 1 vial at 10 mL
For in vitro use only.
Specifications
LacBuster™-L is a novel β-lactamase formulation with a broad substrate range against β-lactam antibiotics including carbapenems, cephalosporins up to 5th
generation and penicillins. LacBusterTM-L is available as a ready-to-use sterile solution.
Unit Definition
One International unit of βI activity (Penicillinase) will hydrolyze 1.0 μmole benzylpenicillin per minute at pH 7.0 and 25°C. One IU βI corresponds to 600 Levy
units or 100.000 Kersey Units, respectively.
One International unit of βII activity (Cephalosporinase) will hydrolyze 1.0 μmole cephalosporin C per minute at pH 7.0 and 25°C.
Sterility
LacBuster™-L vials are filled under aseptic conditions.
No growth in Tryptic Soy broth is observed after incubation at 30-35°C for 14 days.
Storage/Stability
LacBuster™-L is stable at 2-8°C for at least one year from the date analysis. At room temperature (15-25°C) LacBuster™-L is stable for at least one month.
Applications
LacBuster™-L can be applied for sterility testing of β- lactam antibiotics according to US and European Pharmacopeia. Due to its high activity, LacBuster™-L is
especially well suited for the direct inoculation method but can be used as well for membrane filtration tests. It can be easily incorporated into existing standard operating procedures by addition of the provided sterile enzyme solution to all buffers and media.
To validate complete inactivation of the β-lactam a challenge test (using less than 100 colony-forming units of
Staphylococcus aureus ATCC 6538 or Bacillus subtilis ATCC 6633) with an appropriate quantity of enzyme has to be performed under the custom
specific settings. Please refer to our application specialists for further advice.
Further applications include environmental monitoring of manufacturing areas and sterility testing of blood cultures. For preparation of agar plates, LacBuster™-L is added to the medium at a temperature of ≤ 50°C.
Substrate Range
LacBuster™-L has been demonstrated to inactivate following β-lactam antibiotics efficiently:
• Carbapenems: Doripenem, Ertapenem, Imipenem, Meropenem
• Cephalosporins: Cefaclor, Cefadroxil, Cefazolin, Cefepime, Cefixime, Cefotaxime, Cefoxitin, Cefpodoxime, Ceftazidime, Ceftibuten, Ceftriaxon, Cefuroxime, Cephalexin
• Penicillins: Amoxicillin, Ampicillin, Benzylpenicillin, Cloxacillin, Nafcillin, Oxacillin, Piperacillin, Ticarcillin
This product was originally developed by EUCODIS Bioscience GmbH, now Biosynth.
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| Brand | Pyrex |