LacBuster-S 50

Lacbuster S-50

Formulation: freeze-dried powder
Sales Unit: 10 vials
Activity: >500 IU BI, >50 IU BII per vial
Store at 5°C
For in vitro use only.

Specifications
LacBuster™-S is a ß-lactamase formulation with a broad substrate range against ß-lactam antibiotics including carbapenems, cephalosporins up to 5 generation and
penicillins.
LacBuster™-S is available as a freeze-dried product containing buffer salts (potassium phosphate, sodium chloride and sorbitol).

Unit definition
One International unit of Penicillinase activity (IU ß-I) will hydrolyze 1.0 μmole benzylpenicillin per minute at pH 7.0 and 25°C. One IU ß-I corresponds to 600 Levy units or 100.000 Kersey Units, respectively.
One International unit of Cephalosporinase activity (IU ß-II) will hydrolyze 1.0 μmole cephalosporin C per minute at pH 7.0 and 25°C.

Sterility
LacBuster™-S vials are sterilised by gamma-irradiation.
There is no detectable growth in Tryptone Soy broth at 30-35°( for 14 days.

Storage/ Stability
LacBuster™-S is stable in unopened vials for 2 years from the date of analysis at 2-8°C. After reconstitution
LacBuster™-S is stable for 2 months at 2-8°C

Application

LacBuster™-S can be applied for sterility testing of ß-lactam antibiotics by membrane filtration according to US and European Pharmacopeia methods. It can be easily incorporated into existing standard operating procedures by addition of the reconstituted sterile-filtered enzyme solution to all buffers and media.
To validate complete inactivation of the ß-lactam a challenge test (using less than 100 colony-forming units of Staphylococcus aureus ATCC 6538 or Bacillus subtilis ATCC 6633) with an appropriate quantity of enzyme has to be performed under the customer-specific settings. Please refer to our application specialists for further advice.
Further applications include environmental monitoring of manufacturing areas and sterility testing of blood cultures.

Recommendations for use
For reconstitution, we recommend dissolving the content of one vial EBL021.2 in 10 ml of deionized, purified water.
After reconstitution, filter-sterilize through a 0.2 μm low- protein binding membrane.
For preparation of agar plates, LacBuster™-S should be added to the medium at a temperature of < 55"C.

Substrate Range
LacBuster™-S has been demonstrated to inactivate following ß-lactam antibiotics efficiently:
• Cephalosporins: Cefaclor, Cefadroxil, Cefazolin, Cefepime, Cefixime, Cefotaxime, Cefoxitin, Cefpodoxime, Ceftazidime, Ceftibuten, Ceftriaxon, Cefuroxime, Cephalexin
• Carbapenems: Doripenem, Ertapenem, lmipenem, Meropenem
• Penicillins: Amoxicillin, Ampicillin, Benzylpenicillin, Oxacillin, Piperacillin. Ticarcillin

This product was originally developed by EUCODIS Bioscience GmbH, now Biosynth.